DPB1 T-Cell Epitope Algorithm v2.0

Classification of HLA-DPB1 mismatches based on T-cell-epitope Groups (TCE-Groups) has been shown to identify mismatches that might be tolerated (permissive) and those that would increase risks (non-permissive) after unrelated-donor haematopoietic stem cell transplantation (HSCT). This calculator allows you to enter the HLA-DPB1 typing of a patient and donor and view the predicted T-Cell epitopes and resulting prediction of the effect of mismatching when selecting appropriate donors for HSCT recipients.

Prospective HLA-DPB1 Typing
 DPB1*  DPB1* 
 DPB1*  DPB1* 
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Please enter a valid DPB1 typing using the numerical allele code only, for example DPB1*01:01:01 would be entered as '01:01:01'. Ambiguous typings are not permitted and all alleles should be defined to at least two fields; i.e. '01:01' and not '01'. In the case of null alleles or singele typings, these will be treated as homozygous for the expressed allele.

The tool has been updated following the recent publication;

  • Crivello P, Zito L, Sizzano F, et al.
    The Impact of Amino Acid Variability on Alloreactivity Defines a Functional Distance Predictive of Permissive HLA-DPB1 Mismatches in Hematopoietic Stem Cell Transplantation
    Biol Blood Marrow Transplant (2015) 21:233-41.

The TCE group assignment is reported for all DPB1 alleles according to Release 3.38.0 of the IPD-IMGT/HLA Database, released 2019-10.

The following syntax is used:

  • The suffix 'a' is added to the TCE group of 4 alleles to indicate that they were reclassified in the Crivello et al. 2015 paper. These alleles are DPB1*06:01, DPB1*08:01, DPB1*19:01 and DPB1*106:01.
  • The suffix '$' is added to the TCE group to indicate those alleles that were assigned by functional distance scores only.

The implementation of the DPB1 T-Cell Epitope algorithm has been written in collaboration with Katharina Fleischhauer, University Hospital Essen, Germany and Bronwen Shaw, CIBMTR, USA. Regular updates of the DPB1 T-Cell Epitope algorithm with new alleles are performed in collaboration with Pietro Crivello, University Hospital Essen, Germany.

Disclaimer - This tool is being offered as a tool to predict T-Cell epitope matching at DPB1 as reported in:

  • Crivello P, Zito L, Sizzano F, et al.
    The Impact of Amino Acid Variability on Alloreactivity Defines a Functional Distance Predictive of Permissive HLA-DPB1 Mismatches in Hematopoietic Stem Cell Transplantation
    Biol Blood Marrow Transplant (2015) 21:233-41.
  • Fleischhauer K, Shaw BE, Gooley T, et al.
    Effect of T-cell-epitope matching at HLA-DPB1 in recipients of unrelated-donor haemopoietic-cell transplantation: a retrospective study.
    Lancet Oncology (2012) 13:366-74
  • Crocchiolo R, Zino E, Vago L, et al.
    Nonpermissive HLA-DPB1 disparity is a significant independent risk factor for mortality after unrelated hematopoietic stem cell transplantation.
    Blood (2009) 114:1437-44.
  • Zino E, Vago L, Di Terlizzi S, et al.
    Frequency and targeted detection of HLA-DPB1 T cell epitope disparities relevant in unrelated hematopoietic stem cell transplantation.
    Biol Blood Marrow Transplant (2007) 13:1031-40.
  • Zino E, Frumento G, Marktel S, et al.
    A T-cell epitope encoded by a subset of HLA-DPB1 alleles determines nonpermissive mismatches for hematologic stem cell transplantation.
    Blood (2004) 103:1417-24.

No information entered into this tool is collected or stored on our servers.

API

An API has been developed for this tool, please see our API Documentation for further details